Alternative Splicing Tool

The tool analyzes alternative splicing by ingesting isoform-level counts or transcript abundances across samples. It computes PSI values for exon inclusion events and outputs per-event metrics that reveal isoform usage patterns.
Conceptually, the tool relies on a standard inclusion/exclusion model, supporting multi-isoform events, normalization, and species-specific annotations. It can compare conditions to identify differential splicing with delta PSI and simple heuristics, while maintaining reproducible workflows.
Beneficiaries include molecular biologists, computational biologists, and labs performing RNA-seq analysis or transcriptomics studies. Unique value lies in transparent calculations, auditable pipelines, and ready exportable reports compatible with downstream analyses.
Core features include PSI calculation, per-event reporting, event filtering, sample metadata handling, and export in CSV/JSON. Use cases span tissue-specific splicing, treatment response, and isoform switch investigations; the tool emphasizes robust validation and clear assumptions.