PsRNATarget Web Tool

The PsRNATarget Web Tool analyzes plant RNA sequences to estimate accessibility and guide design decisions. It accepts user-provided transcript sequences and species identifiers, then computes a site-level accessibility score and flags high-potential target regions for silencing or editing.
Conceptual approach: The tool applies lightweight heuristics to approximate RNA accessibility, combining sequence features, GC balance, and a simple secondary-structure proxy. It performs optional off-target screening against a user-provided reference set and returns per-site scores and a concise summary.
Who benefits: plant scientists, educators, biotech teams; use cases include selecting siRNA/shRNA targets, designing guide RNAs for CRISPR-based transcript targeting, and creating teaching datasets. Unique value includes fast local accessibility scoring without requiring external data, batch-ready export, and pipeline-friendly outputs.
Core features & use cases: Inputs include sequence, species, and optional reference set; Outputs include accessibility_score (0-1), recommended_positions, gc_content, and a CSV/JSON export; Advanced features include adjustable window size, weights for scoring, and API access for automation.