15N Tracing Analyzer

This tool analyzes isotopic tracer data from 15N experiments to provide corrected enrichment metrics and, optionally, simple flux estimates. It targets researchers in metabolomics, biochemistry, and systems biology who need transparent, reproducible analysis of isotope labeling data when designing or interpreting tracer experiments.
Conceptually, it parses a tabular dataset, applies natural abundance correction on 15N signals, converts to fractional enrichment, and aggregates results by metabolite and time point. When a network of metabolites is provided via inputs or assumed, it estimates fluxes using a simple mass-balance model and least-squares minimization; uncertainty can be approximated via bootstrap resampling.
Core features include data ingestion; 15N correction; fractional enrichment calculation; optional flux estimation; exportable results; and support for multiple timepoints. The tool uses standard isotopic definitions and SI units, enabling cross-lab use and reproducible documentation.
Use cases include tracking label incorporation over time, comparing conditions, and validating pathway activity. It differentiates by offering an integrated correction and minimal modeling pipeline suitable for publication-ready figures and reproducible analyses.