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PCR Tool

Simulate PCR reactions with this free online tool. Calculate melting temperatures, product length, and visualize DNA amplification.

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About This Tool

This online PCR tool allows you to simulate Polymerase Chain Reaction (PCR) experiments digitally. Input your DNA template sequence, forward and reverse primers, and adjust PCR parameters like annealing temperature and cycle count. The tool calculates primer melting temperatures using the nearest-neighbor method, predicts product length, and simulates amplification. Results include a detailed breakdown of the amplified sequence, melting curves, and product characteristics. Perfect for students, researchers, and educators to plan and validate PCR experiments without lab equipment.
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How to Use

  1. Enter a DNA template sequence (e.g., ATGCGTA...).
  2. Input forward and reverse primer sequences.
  3. Adjust PCR parameters like annealing temperature and cycles.
  4. Click 'Simulate PCR' to run the simulation.
  5. View results including product sequence, melting temperature, and length.
How to use online pcr tool

Frequently Asked Questions

Find Quick Answers

What is PCR and how does this tool help?
PCR (Polymerase Chain Reaction) is a lab technique to amplify DNA. This tool simulates PCR digitally, helping you plan experiments by calculating melting temperatures and predicting products.
How accurate is the melting temperature calculation?
The tool uses the nearest-neighbor method with salt correction, which is standard for primer design. It's accurate for typical PCR conditions but consult lab protocols for critical applications.
Can I use degenerate bases in primers?
Yes, the tool supports standard IUPAC codes for degenerate bases (e.g., R for A/G, Y for C/T). It will calculate an average melting temperature.
What happens if primers don't match the template?
The tool validates primer binding. If mismatches are detected, it shows an error with details to help you adjust sequences.

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