DNA Chisel

Optimize DNA sequences for expression, stability, and cloning with multiple parameters.

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6,700+

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< 15ms

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100%

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About This Tool

DNA Chisel is a versatile sequence optimization tool that allows researchers to optimize DNA sequences for various applications. It performs codon optimization for expression in different organisms, adjusts GC content, removes unwanted restriction sites, and predicts secondary structures. The tool provides visual feedback with color-coded sequences and detailed optimization reports.

Save your calculation history with a free account

How to Use

Paste your DNA sequence in the input area
Select optimization parameters (codon usage, GC content range, restriction sites to avoid)
Click 'Optimize Sequence' to process
View the optimized sequence with highlighted changes and detailed statistics
Copy the optimized sequence or download it as a FASTA file

Frequently Asked Questions

Find Quick Answers

What DNA sequence formats are supported?

The tool accepts raw DNA sequences (ATCG characters), FASTA format, or GenBank format. Non-DNA characters are automatically filtered out.

How does codon optimization work?

Codon optimization replaces rare codons with more frequent ones for your selected organism (E. coli, yeast, human, etc.) to improve protein expression levels.

Can I exclude specific restriction sites?

Yes, you can specify restriction enzyme names or recognition sequences to avoid in the optimized sequence.

Is there a limit on sequence length?

The tool can handle sequences up to 10,000 base pairs. For longer sequences, consider breaking them into smaller fragments.

Need to run multiple calculations?

Create a free account today to unlock unlimited daily runs, access advanced parameters, save your history, and request custom features.

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