Illumina Coverage Calculator

Estimate sequencing coverage for Illumina platforms quickly and accurately to optimize your genomics experiments and data quality.

Illustration of DNA sequencing coverage calculatorA modern flat design SVG representing DNA strands, sequencing reads, and coverage graph for Illumina sequencing calculations.

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About This Tool

The Illumina Coverage Calculator is an essential tool for genomics researchers and laboratory professionals. It helps determine how much sequencing data is required to achieve desired coverage depths across genomes or specific target regions. By inputting basic parameters such as genome size, read length, and target coverage, users receive precise calculations of total reads or bases needed. This facilitates efficient experimental design, cost estimation, and quality assurance before sequencing begins.

Designed for ease of use, the calculator provides instant feedback with clear, actionable results. Perfect for planning whole-genome, exome, or targeted sequencing projects on Illumina platforms. Whether optimizing for clinical accuracy or research throughput, this tool ensures your sequencing efforts are both effective and economical.

Additional features include optional error rate adjustments, multi-sample estimates, and result exports. The intuitive interface makes it suitable for scientists, bioinformaticians, and lab technicians alike, providing critical insights to make informed decisions about sequencing depth, resource allocation, and experiment success.

Use the Illumina Coverage Calculator to streamline your workflow, reduce costs, and improve data quality in genomics research and clinical diagnostics.

How to Use

1. Enter your genome size in base pairs.
2. Input your desired coverage depth.
3. Specify the read length used in sequencing.
4. Optionally, adjust error rate or target region size.
5. Click Calculate to view results.
6. Use the export or copy options to share or save your data.

FAQs/Additional Resources

Find Quick Answers

What is sequencing coverage and why is it important?

How does the calculator account for error rates?

Can I calculate for targeted regions or exomes?

Is this calculator suitable for all Illumina platforms?

Can I export my calculation results?

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