AB1 Viewer

The AB1 Viewer provides a focused, non-editing view of Sanger sequencing data stored in AB1 trace files. It decodes raw channel data (A, C, G, T) and exposes per-base calls along with peak intensities to assess read quality. By mapping peaks to bases, users can quickly gauge sequence reliability and identify ambiguous regions. The tool is designed for researchers, students, and QA teams who need a fast, repeatable view of trace information without deploying full-spectrum bioinformatics software. Core capabilities include parsing AB1 files, extracting base calls, presenting chromatogram representations, and enabling export of per-base data for downstream workflows. The design emphasizes deterministic outputs and compatibility with simple text formats to support reproducible QC. Use cases include confirming base calls before alignment, validating sequencing runs, and preparing data for quick checks in collaborative projects. Unique value lies in combining rapid AB1 decoding with straightforward exports and a lightweight viewing experience, reducing time-to-insight in sequencing QC and educational settings. Core features cover input validation, trace data extraction, base calling visualization, and export options for CSV or FASTA-like formats. In practice, a lab uploads an AB1 file, inspects the trace and calls, optionally exports the base data, and uses the results to decide if resequencing is warranted or if downstream pipelines can proceed.