Pooling Calculator

About This Tool

This pooling calculator is designed for genomic researchers and biotech professionals who perform Illumina sequencing. By inputting parameters such as the total number of samples, preferred sequencing platform, desired coverage depth, and multiplexing limits, users receive optimized pooling strategies that improve resource allocation. The tool simplifies complex calculations involved in sequencing pool design, factoring in platform-specific characteristics and constraints. It supports quick decision-making, cost estimation, and planning for large-scale genomic projects. Advanced options include accounting for DNA fragment sizes and index hopping risks, giving researchers detailed insights into their sequencing setups and efficiencies. Whether managing small or large sample cohorts, this calculator ensures precise, effective pooling—saving time, reducing costs, and increasing data quality.

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